https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33417 Wed 31 Oct 2018 15:05:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34580 Helicteres hirsuta Lour. (H. hirsuta L.) has been used as a traditional medicine for treatment of various ailments such as malaria and diabetes in Southeast Asian countries. This study aimed to optimize the conventional extraction conditions for obtaining the highest levels of phenolics, flavonoids, and antioxidant activities within H. hirsuta L. using response surface methodology. The Box–Behnken design was employed to evaluate the effects of extraction time, temperature, and the sample‐to‐solvent ratio on bioactive compound yield and antioxidant capacity of H. hirsuta L. The results indicated that the sample‐to‐solvent ratio had the strongest impact on bioactive compounds and antioxidant power of H. hirsuta L. and revealed the optimum extraction conditions to include temperature of 60 °C, time of 35 min at the sample‐to‐solvent ratio of 1 : 100 g/mL using 40% (v/v) methanol as the solvent. Under these conditions, the highest levels of total phenolic content and total flavonoid content were 16.87 mg GAE/g and 17.55 mg CE/g, respectively, significantly higher than the values obtained using water as the solvent. When preparing powered crude extract from H. hirsuta L., the ratio of 3 : 100 (g/mL) was suggested for the saving of 67% of solvent volume and energy required for solvent removal, as similar total phenolic content and total flavonoid content yield (86–87%) as well as antioxidant activities were obtained in comparison with the ratio of 1 : 100 (g/mL).]]> Wed 09 Feb 2022 15:55:50 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33964 Helicteres hirsuta Lour. (H. hirsuta), which has been used as a traditional medicine for malaria and diabetes treatment. Results showed that either ultrasonic temperature or sample-to-solvent ratio had a greater influence on extraction efficiency of total phenolics, flavonoids and antioxidant capacity than that of methanol concentration. Optimal extraction conditions were determined to be ultrasonic temperature of 60 °C, ultrasonic time of 25 min, ultrasonic power of 150W, sample-to-solvent ratio of 3:100 g/mL, and a solvent composition of 40% (v/v) aqueous methanol. Under these conditions, 15.97 mg GAE/g of phenolics, 16.42 mg CE/g of flavonoids, and 13.34 g/100 g of extractable solids were obtained. The highest values for DPPH, ABTS and FRAP assays were also obtained under these conditions, with the exception of CUPRAC (88% maximum value). These optimal extraction conditions can be applied to prepare powdered crude extract for further isolation and purification of individual bioactive compounds for potential use in the pharmaceutical industry.]]> Wed 09 Feb 2022 15:54:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34077 Helicteres hirsuta Lour. The results revealed that all the three powdered extracts from the leaves and the stems possessed high levels of phenolics (177.07–241.03 mg GAE g⁻¹), flavonoids (158.03–280.06 mg CE g⁻¹) and saponins (165.77–1035.33 mg ESE g⁻¹) and exhibited strong antioxidant capacity. HPLC analysis identified nine major compounds in the leaf powder crude extract; however, the leaf aqueous fraction had three extra compounds; whereas, the saponin-enriched butanol leaf fraction had seven extra compounds. For the stems, twelve main compounds were evident in either the powdered crude extract or the aqueous fraction, and five new compounds were revealed in the saponin-enriched butanol fraction. The findings revealed that the powdered aqueous fractions and saponin-enriched butanol fractions are potential sources of biologically active compounds for further investigation and industrial utilisation.]]> Wed 06 Feb 2019 14:24:17 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32046 p‐coumaric acid content. The highest gallic acid content was recorded in the pomace dried by hot air at 110°C. The results of this study indicate that drying technique should be carefully selected according to the bioactive compounds aimed to be extracted.]]> Tue 24 Apr 2018 15:09:46 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43338 Thu 15 Sep 2022 15:04:25 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31497 Sat 24 Mar 2018 08:44:08 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1180 Sat 24 Mar 2018 08:28:28 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20556 Sat 24 Mar 2018 08:02:35 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29664 −1. Under these conditions, the aqueous extracts had the same content of TPC and TF as well as antioxidant capacity in comparison with those of methanol extracts obtained by sonication. Therefore, these conditions could be applied for further extraction and isolation of phenolic compounds from lemon pomace]]> Sat 24 Mar 2018 07:32:23 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34250 75% cure rate following chemotherapy; there is an increasing population of survivors who live with chronic bone defects. Studies suggest that these defects are the result of reduced bone from increased marrow fat formation and increased bone resorption following chemotherapy. These changes probably result from altered expression/activation of regulatory molecules or pathways regulating skeletal cell formation and activity. Treatment with methotrexate, an antimetabolite commonly used in childhood oncology, has been shown to increase levels of proinflammatory/pro-osteoclastogenic cytokines (e.g., enhanced NF-κB activation), leading to increased osteoclast formation and bone resorption, as well as to attenuate Wnt signaling, leading to both decreased bone and increased marrow fat formation. In recent years, understanding the mechanisms of action and potential health benefits of selected nutraceuticals, including resveratrol, genistein, icariin, and inflammatory fatty acids, has led to preclinical studies that, in some cases, indicate efficacy in reducing chemotherapy-induced bone defects. We summarize the supporting evidence.]]> Fri 22 Feb 2019 16:56:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47212 Fri 16 Dec 2022 10:09:29 AEDT ]]>